Gt(ROSA)26Sor^{tm8(CAG-CHRM3*)Jcbr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm8(CAG-CHRM3*)Jcbr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Jens C Bruning
• MGI ID: MGI:7485775
• Synonyms: ROSA26CAGSloxSTOPloxhMD3Gq
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm8(CAG-CHRM3*)Jcbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234407
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm8(CAG-CHRM3*)Jcbr} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CHRM3 (1131)

  Chrm3

  (MGI:88398)

  Inserted sequence harbor two amino acid substitutions (Y149C and A239G) that abolish receptor affinity for the native ligand

• Gt(ROSA)26Sor^{tm8(CAG-CHRM3*)Jcbr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The targeting construct contained the CAG promoter (chicken beta actin promoter with CMV enhancers), a floxed neomycin-Westphal stop sequence (WSS) cassette, and a modified Gq-coupled human M3 muscarinic receptor (CHRM3) gene. The wild-type human muscarinic 3 receptor sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y149C and A239G) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:234407)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:234407 Steculorum SM, et al., AgRP Neurons Control Systemic Insulin Sensitivity via Myostatin Expression in Brown Adipose Tissue. Cell. 2016 Mar 24;165(1):125-38
• All: 8 reference(s)