Unc13a^tm6Bros
Targeted Allele Detail

Summary

• Symbol: Unc13a^tm6Bros
• Name: unc-13 homolog A; targeted mutation 6, Nils Brose
• MGI ID: MGI:7484680
• Synonyms: C2BKW
• Gene: Unc13a Location: Chr8:72079356-72124418 bp, - strand Genetic Position: Chr8, 34.43 cM, cytoband B3.3
• Alliance: Unc13a^tm6Bros page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325344
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Lysine codon 702 (AAG) in exon 18 was changed to tryptophan (TGG) (p.K702W) and an FRT site flanked puromycin resistance gene cassette was inserted into intron 17. The puro cassette was removed through subsequent Flp-mediated recombination. This mutation is located in the C2B domain of the encoded polypeptide and enhances Ca²⁺-dependent phospholipid binding by the C2B domain without changing the Ca²⁺ affinity. (J:325344)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Unc13a Mutation: 75 strains or lines available

References

• Original: J:325344 Lipstein N, et al., Munc13-1 is a Ca(2+)-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission. Neuron. 2021 Dec 15;109(24):3980-4000.e7
• All: 1 reference(s)