Mustn1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mustn1^{tm1c(EUCOMM)Wtsi}
• Name: musculoskeletal, embryonic nuclear protein 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7484529
• Gene: Mustn1 Location: Chr14:30601214-30603567 bp, + strand Genetic Position: Chr14, 19.09 cM
• Alliance: Mustn1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mustn1 gene page

Mutation origin

• Mutant Cell Line: EPD0917_3_F04
• Germline Transmission: Earliest citation of germline transmission: J:335748
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 30602117 of Chromosome 14 upstream of the critical exon 2 and part of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 and part of exon 3 at position 30603225. The critical exon 2 and part of exon 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:335748)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mustn1 Mutation: 7 strains or lines available

References

• Original: J:335748 Kim CJ, et al., Mustn1 ablation in skeletal muscle results in increased glucose tolerance concomitant with upregulated GLUT expression in male mice. Physiol Rep. 2023 May;11(9):e15674
• All: 2 reference(s)