Mgp^em2Murs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mgp^em2Murs
• Name: matrix Gla protein; endonuclease-mediated mutation 2, Monzur Murshed
• MGI ID: MGI:7482790
• Synonyms: Mgp^S3mut
• Gene: Mgp Location: Chr6:136849433-136852821 bp, - strand Genetic Position: Chr6, 66.72 cM
• Alliance: Mgp^em2Murs page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codons 22 (TCT), 25 (AGC) and 28 (TCC) were targeted for mutation to alanine codons (GCT, GCC, GCT) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This allele contains all three mutations (p.S22A, p.S25A, p.S28A (p.S3A, p.S6A, p.S9A in mature protein)). The mutations block phosphorylation of the residues in the encoded peptide. (J:326013)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mgp Mutation: 13 strains or lines available

References

• Original: J:326013 Parashar A, et al., Prevention of Arterial Elastocalcinosis: Differential Roles of the Conserved Glutamic Acid and Serine Residues of Matrix Gla Protein. Arterioscler Thromb Vasc Biol. 2022 Jun;42(6):e155-e167
• All: 1 reference(s)