Npr2^tm1.2Laj
Targeted Allele Detail

Summary

• Symbol: Npr2^tm1.2Laj
• Name: natriuretic peptide receptor 2; targeted mutation 1.2, Laurinda A Jaffe
• MGI ID: MGI:7482668
• Synonyms: Npr2-7E cKI
• Gene: Npr2 Location: Chr4:43631935-43651244 bp, + strand Genetic Position: Chr4, 23.05 cM
• Alliance: Npr2^tm1.2Laj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129 x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert a loxP site, a a wild-type Npr2 minigene with 200 bp of intron 8 and cDNA from exons 8-22 (from BAC vector, RP24-306K11 and full length Npr2 cDNA), an IRES/EGFP reporter, an FRT-flanked PGK neomycin cassette and a loxP site into intron 7 and insert the 7E point mutations into exons 8 and 9. The 7E point mutations consist of 7 glutamate substitutions: S489E, S513E, T516E, S518E, S523E, S526E, T529E. Flp-mediated recombination removed the neo cassette. (J:228790)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Npr2 Mutation: 61 strains or lines available

References

• Original: J:228790 Shuhaibar LC, et al., Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase is required for rapid resumption of oocyte meiosis in response to luteinizing hormone. Dev Biol. 2016 Jan 1;409(1):194-201
• All: 3 reference(s)