Dnmt3a^{tm1(DNMT3A*R882H)Mbp}
Targeted Allele Detail

Summary

• Symbol: Dnmt3a^{tm1(DNMT3A*R882H)Mbp}
• Name: DNA methyltransferase 3A; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:7469610
• Synonyms: DNMT3A^fl-R882H-fl
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^{tm1(DNMT3A*R882H)Mbp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334992
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutation: Insertion
• Mutation details: A loxp-STOP-loxP neomycin resistance cassette, exon 1 of mouse Dnmt3a, and human DNMT3A cDNA containing an arginine to histidine substitution at residue 882 (CGC to CAC) replaced exon 1. (J:334992)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 140 strains or lines available

References

• Original: J:334992 Scheller M, et al., Hotspot DNMT3A mutations in clonal hematopoiesis and acute myeloid leukemia sensitize cells to azacytidine via viral mimicry response. Nat Cancer. 2021 May;2(5):527-544
• All: 3 reference(s)