Twist1^em1Wdf
Endonuclease-mediated Allele Detail

Summary

• Symbol: Twist1^em1Wdf
• Name: twist basic helix-loop-helix transcription factor 1; endonuclease-mediated mutation 1, Walid D Fakhouri
• MGI ID: MGI:7469499
• Synonyms: Twist1^S18I;20A
• Gene: Twist1 Location: Chr12:34007670-34009828 bp, + strand Genetic Position: Chr12, 14.81 cM, cytoband B-C1
• Alliance: Twist1^em1Wdf page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codon 18 (AGC) was changed to isoleucine (ATC) (p.S18I) and serine codon 20 (AGC) to alanine (GCT) (p.S20A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. These mutations prevent phosphorylation of the highly conserved residues. (J:327336)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Twist1 Mutation: 20 strains or lines available

References

• Original: J:327336 Bertol JW, et al., TWIST1 interacts with beta/delta-catenins during neural tube development and regulates fate transition in cranial neural crest cells. Development. 2022 Aug 1;149(15):dev200068
• All: 1 reference(s)