Idh1^tm1.3Crisp
Targeted Allele Detail

Summary

• Symbol: Idh1^tm1.3Crisp
• Name: isocitrate dehydrogenase 1 (NADP+), soluble; targeted mutation 1.3, Cristina Pina
• MGI ID: MGI:7468731
• Synonyms: Idh1^mut, Idh1^R132H-KI
• Gene: Idh1 Location: Chr1:65197775-65225638 bp, - strand Genetic Position: Chr1, 32.91 cM
• Alliance: Idh1^tm1.3Crisp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327421
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site, engrailed 2 (En2) splice acceptor sequence, cDNA for Idh1 exons 3-9, SV40 poly(A) signal sequences, a second loxP site, an FRT site, a neomycin resistance gene cassette and a second FRT site were inserted into intron 2 and a p.R132H (arginine 132 to histidine) mutation was introduced in exon 3. The neo cassette was removed through subsequent Flp-mediated recombination and the cDNA was removed through Cre-mediated recombination. (J:327421)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Idh1 Mutation: 41 strains or lines available

References

• Original: J:327421 Gupta S, et al., Transcriptional variability accelerates preleukemia by cell diversification and perturbation of protein synthesis. Sci Adv. 2022 Aug 5;8(31):eabn4886
• All: 1 reference(s)