Gt(ROSA)26Sor^{em1.1(CAG-EGFP/Rpl10)Mgmj}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-EGFP/Rpl10)Mgmj}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Martin G Myers, Jr
• MGI ID: MGI:7467072
• Synonyms: Rosa26^{RFL-eGFP-L10a}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-EGFP/Rpl10)Mgmj} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The allele is generateb from Gt(ROSA)26Sor^{em1(CAG-EGFP/Rpl10)Mgmj} by germline deletion of the lox-stop-lox cassette. Using CRISPR/Cas9 technology, a targeting vector CAG-FSF-LSL followed by an EGFP/Rpl10 fusion reporter gene under the control of the CAG promoter (CVM enhancer/chicken ACTB promoter) was inserted into the Gt(ROSA)26Sor locus. The lox-stop-lox cassette (LSL) was removed by crossing to a germ line cre-deleter. It is an Flp-dependent reporter. The expression of the EGFP/Rpl10 fusion reporter gene requires the removal of FSF by Flp recombinases. (J:317381)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:317381 Sabatini PV, et al., tTARGIT AAVs mediate the sensitive and flexible manipulation of intersectional neuronal populations in mice. Elife. 2021 Mar 11;10:e66835
• All: 2 reference(s)