Gt(ROSA)26Sor^{em1(CAG-EGFP/Rpl10)Mgmj}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-EGFP/Rpl10)Mgmj}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Martin G Myers, Jr
• MGI ID: MGI:7467071
• Synonyms: Rosa26^{RCFL-eGFP-L10a}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-EGFP/Rpl10)Mgmj} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, a targeting vector CAG-FSF-LSL followed by an EGFP/Rpl10 fusion reporter gene under the control of the CAG promoter (CVM enhancer/chicken ACTB promoter) was inserted into the Gt(ROSA)26Sor locus. It is an Flp- and Cre-dependent reporter. The expression of the EGFP/Rpl10 fusion reporter gene requires the removal of both LSL by Cre and FSF by Flp recombinases. (J:317381)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1042 strains or lines available

References

• Original: J:317381 Sabatini PV, et al., tTARGIT AAVs mediate the sensitive and flexible manipulation of intersectional neuronal populations in mice. Elife. 2021 Mar 11;10:e66835
• All: 3 reference(s)