Tg(Grik4-Lgi1)#Mafu
Transgene Detail

Summary

• Symbol: Tg(Grik4-Lgi1)#Mafu
• Name: transgene insertion, Masaki Fukata
• MGI ID: MGI:7466732
• Synonyms: KA1-Lgi1
• Transgene: Tg(Grik4-Lgi1)#Mafu Location: unknown
• Alliance: Tg(Grik4-Lgi1)#Mafu page

Transgene origin

• Strain of Origin: (C57BL/6 X DBA/2)F1 x C57BL/6

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Grik4-Lgi1)#Mafu expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Lgi1 (252892)

  Lgi1	(MGI:1861691)

  mouse promoter, rat cDNA with C-terminal FLAG and 6xHis tags

• Mutation details: The transgene insert comprises mouse BAC RP23-353O19 where the rat Lgi1 cDNA (with sequence for a C-terminal FLAG tag and 6xHis tag inserted immediately upstream of the stop codon) and a kanamycin resistance gene were inserted immediately downstream of the Lgi1 ATG translation start site. (J:328338)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:328338 Yokoi N, et al., 14-3-3 proteins stabilize LGI1-ADAM22 levels to regulate seizure thresholds in mice. Cell Rep. 2021 Dec 14;37(11):110107
• All: 1 reference(s)