Mtor^em1Dcf
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mtor^em1Dcf
• Name: mechanistic target of rapamycin kinase; endonuclease-mediated mutation 1, Diane C Fingar
• MGI ID: MGI:7466519
• Synonyms: mTOR^A
• Gene: Mtor Location: Chr4:148533068-148642140 bp, + strand Genetic Position: Chr4, 78.76 cM, cytoband E1
• Alliance: Mtor^em1Dcf page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: Serine codon 2159 (TCT) in exon 46 was changed to alanine (GCT) (p.S2159A) using two sgRNAs (targeting CACCGTGGTTGGGGTCGTATGTTCC and AAACGGAACATACGACCCCAACCAC) and an ssODN template (GTTCAAAGCTCACATCCCTGGAGCTGCAGTATGTGTCCCCCAAACTTCTGATGTGCCGAGACCTTGAGTTGGCTGTGCCCGGGACATATGACCCAAACCAGCCAATCATTCGCATTCAATCCATAGCCCCGGCTTTGCAAGTCATCACATCCAAGCAGAGGCCTCGGAAGCTGACTCTGA) with CRISPR/Cas9 technology. This mutation prevents phosphorylation of the residue. (J:257513)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtor Mutation: 117 strains or lines available

References

• Original: J:257513 Bodur C, et al., The IKK-related kinase TBK1 activates mTORC1 directly in response to growth factors and innate immune agonists. EMBO J. 2018 Jan 4;37(1):19-38
• All: 2 reference(s)