Ythdc2^em3Rspi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ythdc2^em3Rspi
• Name: YTH domain containing 2; endonuclease-mediated mutation 3, Ramesh S Pillai
• MGI ID: MGI:7466370
• Synonyms: Ythdc2^cat-dead
• Gene: Ythdc2 Location: Chr18:44961521-45022787 bp, + strand Genetic Position: Chr18, 23.81 cM
• Alliance: Ythdc2^em3Rspi page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: Glutamic acid codon 332 (GAA) in exon 7 was changed to glutamine (CAA) (p.E332Q) using crRNA, tracrRNA and an ssODN template with CRISPR/Cas9 technology. This mutation, in the N-terminal RecA module of the RNA helicase domain, inactivates the protein's ATPase and RNA helicase activities. (J:328765)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ythdc2 Mutation: 58 strains or lines available

References

• Original: J:328765 Li L, et al., The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse fertility independently of m(6)A recognition. Mol Cell. 2022 May 5;82(9):1678-1690.e12
• All: 1 reference(s)