Mcph1^tm1.2Litl
Targeted Allele Detail

Summary

• Symbol: Mcph1^tm1.2Litl
• Name: microcephaly, primary autosomal recessive 1; targeted mutation 1.2, Tangliang Li
• MGI ID: MGI:7465046
• Synonyms: Mcph1^deltaex8
• Gene: Mcph1 Location: Chr8:18645147-18853205 bp, + strand Genetic Position: Chr8, 10.24 cM, cytoband A3
• Alliance: Mcph1^tm1.2Litl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328987
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP site was inserted into intron 7 together with a self-excision neomycin cassette flanked by Rox sites and a second loxP site was inserted into intron 8. The neomycin cassette was self-excised. Exon 8 was deleted via cre-mediated recombination in the germline. RT-PCR confirmed the in-frame deletion of the central domain (exon 8). Western blot analysis using an antibody against the middle domain confirmed its absence. (J:328987)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcph1 Mutation: 58 strains or lines available

References

• Original: J:328987 Wang Y, et al., The Central Domain of MCPH1 Controls Development of the Cerebral Cortex and Gonads in Mice. Cells. 2022 Aug 31;11(17)
• All: 1 reference(s)