Cnih3^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cnih3^{tm1d(KOMP)Wtsi}
• Name: cornichon family AMPA receptor auxiliary protein 3; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7461410
• Synonyms: Cnih3 KO
• Gene: Cnih3 Location: Chr1:181180193-181288206 bp, + strand Genetic Position: Chr1, 84.53 cM, cytoband H4
• Alliance: Cnih3^{tm1d(KOMP)Wtsi} page
• IMPC: Cnih3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314957
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 181277051 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 181277890. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:314957)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnih3 Mutation: 16 strains or lines available

References

• Original: J:314957 Frye HE, et al., Sex Differences in the Role of CNIH3 on Spatial Memory and Synaptic Plasticity. Biol Psychiatry. 2021 Dec 1;90(11):766-780
• All: 2 reference(s)