Atp11a^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Atp11a^{tm1b(KOMP)Wtsi}
• Name: ATPase, class VI, type 11A; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7460282
• Gene: Atp11a Location: Chr8:12807016-12918728 bp, + strand Genetic Position: Chr8, 5.73 cM, cytoband A2
• Alliance: Atp11a^{tm1b(KOMP)Wtsi} page
• IMPC: Atp11a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333799
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 12872185 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 12873331. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333799)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp11a Mutation: 53 strains or lines available

References

• Original: J:333799 Radford BN, et al., Defects in placental syncytiotrophoblast cells are a common cause of developmental heart disease. Nat Commun. 2023 Mar 1;14(1):1174
• All: 1 reference(s)