Cpeb3^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cpeb3^{tm1b(EUCOMM)Hmgu}
• Name: cytoplasmic polyadenylation element binding protein 3; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7451383
• Gene: Cpeb3 Location: Chr19:36998691-37186000 bp, - strand Genetic Position: Chr19, 32.08 cM
• Alliance: Cpeb3^{tm1b(EUCOMM)Hmgu} page
• IMPC: Cpeb3 gene page

Mutation origin

• Mutant Cell Lines: HEPD0670_2_C02, HEPD0670_2_G03
• Germline Transmission: Earliest citation of germline transmission: J:288987
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 43401338 of Chromosome 5 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 43402289. The critical exon 3 is thus flanked by loxP sites. Cre-mediated recombination removed the neomycin and exon 3, generating a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:288987)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpeb3 Mutation: 34 strains or lines available

References

• Original: J:288987 Pascual R, et al., The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer. Sci Adv. 2020 May;6(20):eaax3868
• All: 1 reference(s)