Col1a1^{tm29(CAG-Pik3ca*E545K,-luc2)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm29(CAG-Pik3ca*E545K,-luc2)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 29, The Netherlands Cancer Institute
• MGI ID: MGI:7451118
• Synonyms: Col1a1^{invCAG-Pik3caE545K-IRES-Luc}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm29(CAG-Pik3ca*E545K,-luc2)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283289
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: RMCE introduced into the 3' UTR an FRT-flanked cassette containing an inverted lox71/lox66-flanked CAG promoter, full-length Pik3ca sequence with the mutation that results in the amino acid substitution of glutamic acid with lysine, IRES sequence and firefly luciferase reporter gene (fLuc2). (J:283289)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

Mutation created in ES cells containing Cdh1^tm1Jjon and Tg(Wap-cre)51Nki.

References

• Original: J:283289 Wellenstein MD, et al., Loss of p53 triggers WNT-dependent systemic inflammation to drive breast cancer metastasis. Nature. 2019 Aug;572(7770):538-542
• All: 1 reference(s)