Braf^tm2Mmcm
Targeted Allele Detail

Summary

• Symbol: Braf^tm2Mmcm
• Name: Braf transforming gene; targeted mutation 2, Martin McMahon
• MGI ID: MGI:7448917
• Synonyms: Braf^FA
• Gene: Braf Location: Chr6:39580171-39702397 bp, - strand Genetic Position: Chr6, 18.43 cM
• Alliance: Braf^tm2Mmcm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225607
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a FRT site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a PGK-neo STOP cassette and a second FRT site. A modified exon 15 was engineered into the vector to encode Braf with a valine to glutamate substitution at amino acid 600 (p.V600E) and a silent XbaI restriction site. (J:225607)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Braf Mutation: 58 strains or lines available

References

• Original: J:225607 Shai A, et al., TP53 Silencing Bypasses Growth Arrest of BRAFV600E-Induced Lung Tumor Cells in a Two-Switch Model of Lung Tumorigenesis. Cancer Res. 2015 Aug 1;75(15):3167-80
• All: 2 reference(s)