Cited1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cited1^{tm1c(EUCOMM)Hmgu}
• Name: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7448535
• Synonyms: Cited1^loxP
• Gene: Cited1 Location: ChrX:101290987-101295787 bp, - strand Genetic Position: ChrX, 45.25 cM
• Alliance: Cited1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Cited1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334320
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 101290772 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 101291987. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:334320)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cited1 Mutation: 5 strains or lines available

References

• Original: J:334320 Gonzalez-Garcia I, et al., Estradiol regulates leptin sensitivity to control feeding via hypothalamic Cited1. Cell Metab. 2023 Mar 7;35(3):438-455.e7
• All: 1 reference(s)