Nfkb1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nfkb1^{tm1c(KOMP)Wtsi}
• Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7447092
• Synonyms: p50(f/f)
• Gene: Nfkb1 Location: Chr3:135290416-135397308 bp, - strand Genetic Position: Chr3, 62.82 cM
• Alliance: Nfkb1^{tm1c(KOMP)Wtsi} page
• IMPC: Nfkb1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334163
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 135368412 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 135369235. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:334163)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfkb1 Mutation: 104 strains or lines available

References

• Original: J:334163 Barberi T, et al., Absence of host NF-kappaB p50 induces murine glioblastoma tumor regression, increases survival, and decreases T-cell induction of tumor-associated macrophage M2 polarization. Cancer Immunol Immunother. 2018 Oct;67(10):1491-1503
• All: 1 reference(s)