Tn(Pb-Rr388-EGFP)#Zyliu
Transgene Detail

Summary

• Symbol: Tn(Pb-Rr388-EGFP)#Zyliu
• Name: transposon insertion, Zhiyong Liu
• MGI ID: MGI:7447082
• Synonyms: Eh2-Hsp68-EGFP
• Transgene: Tn(Pb-Rr388-EGFP)#Zyliu Location: unknown
• Alliance: Tn(Pb-Rr388-EGFP)#Zyliu page

Mutation origin

• Strain of Origin: CD-1

Mutation description

• Allele Type: Transposon induced (Reporter)
• Mutation: Transposon insertion
• Mutation details: The transgene insert contains the following elements: the 5' PiggyBAC transposon inverted repeat, 1027 bp sequence containing Atoh1 enhancer Eh2 or E3, Hsp68 minimal promoter, EGFP reporter gene, SV40 early poly(A) signal sequences, and the 3' transposon inverted repeat. The transgene was injected into single-cell zygotes together with PiggyBAC transposase mRNA to facilitate integration into the genome. The reporter is expressed in cochlear hair cells in newborn mice. (J:333396)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:333396 Luo Z, et al., Three distinct Atoh1 enhancers cooperate for sound receptor hair cell development. Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2119850119
• All: 1 reference(s)