Gt(ROSA)26Sor^{tm1(CAG-Bcl2)Zhu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Bcl2)Zhu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Yuan Zhang
• MGI ID: MGI:7446375
• Synonyms: R26^LSL-Bcl2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Bcl2)Zhu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334079
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Bcl2)Zhu} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Bcl2 (MGI:88138)

• Mutation details: The targeting vector is constructed based on the Ai6 vector from Addgene with (from 5' to 3') a CAG (CMV enhancer/chicken beta-actin) promoter, an FRT site, a floxed STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), and murine B cell leukemia/lymphoma 2 (Bcl2) cDNA followed by a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. The targeting vector is inserted into the Gt(ROSA)26Sor locus. (J:334079)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:334079 Ratiu JJ, et al., Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-beta selection thymocytes. Nat Commun. 2022 Oct 6;13(1):5901
• All: 1 reference(s)