Zfp384^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zfp384^{tm1c(EUCOMM)Hmgu}
• Name: zinc finger protein 384; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7444924
• Synonyms: Nmp4^fl
• Gene: Zfp384 Location: Chr6:124986108-125014833 bp, + strand Genetic Position: Chr6, 59.17 cM, cytoband F1
• Alliance: Zfp384^{tm1c(EUCOMM)Hmgu} page
• IMPC: Zfp384 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334030
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 125000157 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 125002329. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:334030)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp384 Mutation: 37 strains or lines available

References

• Original: J:334030 Atkinson EG, et al., Conditional Loss of Nmp4 in Mesenchymal Stem Progenitor Cells Enhances PTH-Induced Bone Formation. J Bone Miner Res. 2023 Jan;38(1):70-85
• All: 2 reference(s)