Adora1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Adora1^{tm1c(KOMP)Wtsi}
• Name: adenosine A1 receptor; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7443205
• Synonyms: Adora1^fl, Adora1^loxP
• Gene: Adora1 Location: Chr1:134126963-134163169 bp, - strand Genetic Position: Chr1, 58.15 cM, cytoband E4
• Alliance: Adora1^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0901_5_D12
• Germline Transmission: Earliest citation of germline transmission: J:244455
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 134130433 of Chromosome 1 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 134131847. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene and generated a conditional ready allele. (J:244455)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adora1 Mutation: 23 strains or lines available

References

• Original: J:244455 Blundon JA, et al., Restoring auditory cortex plasticity in adult mice by restricting thalamic adenosine signaling. Science. 2017 Jun 30;356(6345):1352-1356
• All: 2 reference(s)