Setd1a^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Setd1a^{tm1d(EUCOMM)Wtsi}
• Name: SET domain containing 1A; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7442582
• Gene: Setd1a Location: Chr7:127376561-127399294 bp, + strand Genetic Position: Chr7, 69.73 cM
• Alliance: Setd1a^{tm1d(EUCOMM)Wtsi} page
• IMPC: Setd1a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333833
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 127379089 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 127380049. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333833)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Setd1a Mutation: 60 strains or lines available

References

• Original: J:333833 Clifton NE, et al., Developmental disruption to the cortical transcriptome and synaptosome in a model of SETD1A loss-of-function. Hum Mol Genet. 2022 Sep 10;31(18):3095-3106
• All: 2 reference(s)