Htt^tm8(HTT*)Szi
Targeted Allele Detail

Summary

• Symbol: Htt^tm8(HTT*)Szi
• Name: huntingtin; targeted mutation 8, Scott Zeitlin
• MGI ID: MGI:7442513
• Synonyms: Htt^LacQ140, Htt Q175
• Gene: Htt Location: Chr5:34919084-35069878 bp, + strand Genetic Position: Chr5, 17.92 cM
• Alliance: Htt^tm8(HTT*)Szi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333052
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Htt^tm8(HTT*)Szi expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HTT (3064)

  Htt	(MGI:96067)

  expanded CAG repeat 140Q

• Mutation details: The targeting vector was designed to insert a cassette containing synthetic LacO sequences flanking the Htt transcriptional start site followed by a chimeric mouse/human Htt sequence with human exon 1 into the mouse Htt locus on chromosome 5. The human sequence encodes the N terminal sequence including an expanded CAG repeat containing 140Q and the adjacent sequence encoding the human proline-rich region. A loxP-flanked PGK-neomycin cassette was inserted in reverse orientation 1.3 kb upstream of transcription initiation site (J:333052)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Htt Mutation: 179 strains or lines available

References

• Original: J:333052 Marchionini DM, et al., Benefits of global mutant huntingtin lowering diminish over time in a Huntington's disease mouse model. JCI Insight. 2022 Oct 24;7(20)
• All: 1 reference(s)