Map3k20^em1Sbje
Endonuclease-mediated Allele Detail

Summary

• Symbol: Map3k20^em1Sbje
• Name: mitogen-activated protein kinase kinase kinase 20; endonuclease-mediated mutation 1, Simon Bekker-Jensen
• MGI ID: MGI:7442466
• Synonyms: Zak^-
• Gene: Map3k20 Location: Chr2:72115981-72272954 bp, + strand Genetic Position: Chr2, 43.11 cM
• Alliance: Map3k20^em1Sbje page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Not Specified
• Mutation details: CRISPR/Cas9 technology targeting exon 2 generated a frame-shift mutation. Immunoblotting confirmed that both expression of the alpha and beta isoforms was absent in skeletal muscle and liver. (J:333249)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k20 Mutation: 67 strains or lines available

Notes

Strain of Origin information from J:333249 "C57BL/6 X CBA(F1) female mice were used as embryo donors and foster mothers. Super-ovulated female mice (78 weeks old) were mated to males, and fertilized embryos were collected from oviducts."

References

• Original: J:333249 Nordgaard C, et al., ZAKbeta is activated by cellular compression and mediates contraction-induced MAP kinase signaling in skeletal muscle. EMBO J. 2022 Sep 1;41(17):e111650
• All: 6 reference(s)