Ube3b^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ube3b^{tm1c(EUCOMM)Hmgu}
• Name: ubiquitin protein ligase E3B; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7442444
• Synonyms: Ube3b^f
• Gene: Ube3b Location: Chr5:114518668-114559230 bp, + strand Genetic Position: Chr5, 55.99 cM
• Alliance: Ube3b^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ube3b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333720
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 114527986 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 114528771. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333720)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube3b Mutation: 68 strains or lines available

References

• Original: J:333720 Ambrozkiewicz MC, et al., The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc. Mol Psychiatry. 2021 Jun;26(6):1980-1995
• All: 2 reference(s)