Gt(ROSA)26Sor^{tm4(CAG-Calm1*)Bcgen}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-Calm1*)Bcgen}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Biocytogen LLC
• MGI ID: MGI:7442250
• Synonyms: CaMPARI^loxP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-Calm1*)Bcgen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267056
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with a CAG promoter, an FRT site, a loxP-flanked STOP cassette, a Flag-CaMPARI (calcium-modulated photoactivatable ratiometric integrator) sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a poly-A signal, and an attB/att-flanked PGK-FRT-Neo-poly-A cassette. This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation of C57BL/6-derived embryonic stem (ES) cells (J:267056)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:267056 Li X, et al., Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nat Neurosci. 2018 Sep;21(9):1239-1250
• All: 1 reference(s)