Ncf2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ncf2^{tm1d(EUCOMM)Wtsi}
• Name: neutrophil cytosolic factor 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7442034
• Synonyms: Ncf2^-ex3
• Gene: Ncf2 Location: Chr1:152675945-152712742 bp, + strand Genetic Position: Chr1, 65.05 cM
• Alliance: Ncf2^{tm1d(EUCOMM)Wtsi} page
• IMPC: Ncf2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333761
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 152692314 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 152693112. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333761)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncf2 Mutation: 37 strains or lines available

References

• Original: J:333761 Idol RA, et al., Neutrophil and Macrophage NADPH Oxidase 2 Differentially Control Responses to Inflammation and to Aspergillus fumigatus in Mice. J Immunol. 2022 Nov 15;209(10):1960-1972
• All: 1 reference(s)