Fli1^tm1Hill
Targeted Allele Detail

Summary

• Symbol: Fli1^tm1Hill
• Name: Friend leukemia integration 1; targeted mutation 1, Joseph Hill
• MGI ID: MGI:7442022
• Gene: Fli1 Location: Chr9:32333500-32454157 bp, - strand Genetic Position: Chr9, 17.74 cM
• Alliance: Fli1^tm1Hill page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333711
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 32376712 of Chromosome 9 upstream of the exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 3 at position 32377582. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333711)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fli1 Mutation: 26 strains or lines available

References

• Original: J:333711 Ferdous A, et al., Fli1 Promotes Vascular Morphogenesis by Regulating Endothelial Potential of Multipotent Myogenic Progenitors. Circ Res. 2021 Oct 29;129(10):949-964
• All: 1 reference(s)