Map3k8^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Map3k8^{tm1c(KOMP)Wtsi}
• Name: mitogen-activated protein kinase kinase kinase 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7441999
• Synonyms: Map3k8^fl
• Gene: Map3k8 Location: Chr18:4331325-4352978 bp, - strand Genetic Position: Chr18, 2.73 cM
• Alliance: Map3k8^{tm1c(KOMP)Wtsi} page
• IMPC: Map3k8 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:333744
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4341273 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4341273. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:333744)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k8 Mutation: 42 strains or lines available

References

• Original: J:333744 Xie X, et al., TRAF2 regulates T cell immunity by maintaining a Tpl2-ERK survival signaling axis in effector and memory CD8 T cells. Cell Mol Immunol. 2021 Sep;18(9):2262-2274
• All: 1 reference(s)