Nnmt^tm1.2Vlcg
Targeted Allele Detail

Summary

• Symbol: Nnmt^tm1.2Vlcg
• Name: nicotinamide N-methyltransferase; targeted mutation 1.2, Velocigene
• MGI ID: MGI:7439502
• Gene: Nnmt Location: Chr9:48503177-48516453 bp, - strand Genetic Position: Chr9, 26.45 cM
• Alliance: Nnmt^tm1.2Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272957
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 and 1.6 kb upstream of exon 1 was flanked by loxP sites. An FRT-flanked hygromycin selection cassette was inserted downstream of the floxed region and was removed via flp-mediated recombination. Cre-mediated recombination deleted exon 1 in the germline. Western blot analysis confirmed absence of protein in visceral white adipose tissue. (J:272957)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nnmt Mutation: 21 strains or lines available

References

• Original: J:272957 Brachs S, et al., Genetic Nicotinamide N-Methyltransferase (Nnmt) Deficiency in Male Mice Improves Insulin Sensitivity in Diet-Induced Obesity but Does Not Affect Glucose Tolerance. Diabetes. 2019 Mar;68(3):527-542
• All: 1 reference(s)