Bhlhe41^tm1Mbu
Targeted Allele Detail

Summary

• Symbol: Bhlhe41^tm1Mbu
• Name: basic helix-loop-helix family, member e41; targeted mutation 1, Meinrad Busslinger
• MGI ID: MGI:7439464
• Synonyms: Bhlhe41^Tag-neo
• Gene: Bhlhe41 Location: Chr6:145803969-145811146 bp, - strand Genetic Position: Chr6, 77.7 cM, cytoband G2-G3
• Alliance: Bhlhe41^tm1Mbu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258170
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutation: Insertion
• Mutation details: A 217-bp fragment containing the C-terminal tag sequences (fused in frame to the last Bhlhe41 codon) and a 1.9-kb fragment containing the mouse phosphoglycerate kinase (Pgk1) promoter linked to the neomycin-resistance gene (flanked by loxP sites) was inserted into the locus by homologous recombination in ES cells. The tag sequences contained epitopes for antibodies to Flag and V5, two cleavage sites for the TEV protease, and a biotin acceptor sequence (Biotin) for biotinylation by the E. coli biotin ligase BirA. (J:258170)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bhlhe41 Mutation: 12 strains or lines available

References

• Original: J:258170 Kreslavsky T, et al., Essential role for the transcription factor Bhlhe41 in regulating the development, self-renewal and BCR repertoire of B-1a cells. Nat Immunol. 2017 Apr;18(4):442-455
• All: 1 reference(s)