C5ar2^tm1.1Kohl
Targeted Allele Detail

Summary

• Symbol: C5ar2^tm1.1Kohl
• Name: complement component 5a receptor 2; targeted mutation 1.1, Jorg Kohl
• MGI ID: MGI:7432879
• Synonyms: tdTomato-C5ar2^fl
• Gene: C5ar2 Location: Chr7:15968512-15978079 bp, - strand Genetic Position: Chr7, 8.77 cM
• Alliance: C5ar2^tm1.1Kohl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251263
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: Exon 2 was replaced by a cassette encoding a fusion protein composed of tdTomato/C5ar2 cDNA flanked by loxP sites. The self-cleaving peptide P2A was inserted downstream of tdTomato. An frt-flanked neo-pkg was inserted upstream of the tdTomato and was removed via flp-mediated recombination leaving tdTomato and C5ar2 floxed. (J:251263)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C5ar2 Mutation: 20 strains or lines available

References

• Original: J:251263 Karsten CM, et al., Monitoring C5aR2 Expression Using a Floxed tdTomato-C5aR2 Knock-In Mouse. J Immunol. 2017 Nov 1;199(9):3234-3248
• All: 3 reference(s)