Thy1^tm1.1Saal
Targeted Allele Detail

Summary

• Symbol: Thy1^tm1.1Saal
• Name: thymus cell antigen 1, theta; targeted mutation 1.1, Naja Saalbach
• MGI ID: MGI:7432867
• Synonyms: Thy-1^fl
• Gene: Thy1 Location: Chr9:43954681-43959876 bp, + strand Genetic Position: Chr9, 24.52 cM
• Alliance: Thy1^tm1.1Saal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:319158
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 43957515 of Chromosome 9 upstream of the exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 43958419. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:319158)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thy1 Mutation: 212 strains or lines available

References

• Original: J:319158 Schmidt M, et al., Thy-1/beta3 Integrin Interaction-Induced Apoptosis of Dermal Fibroblasts Is Mediated by Up-Regulation of FasL Expression. J Invest Dermatol. 2016 Feb;136(2):526-529
• All: 1 reference(s)