Polr1c^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Polr1c^{tm1c(KOMP)Wtsi}
• Name: polymerase (RNA) I polypeptide C; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7432633
• Synonyms: Polr1c^flx
• Gene: Polr1c Location: Chr17:46554846-46558971 bp, - strand Genetic Position: Chr17, 22.9 cM
• Alliance: Polr1c^{tm1c(KOMP)Wtsi} page
• IMPC: Polr1c gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332795
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 46556374 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 46557340. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:332795)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr1c Mutation: 32 strains or lines available

References

• Original: J:332795 Falcon KT, et al., Dynamic regulation and requirement for ribosomal RNA transcription during mammalian development. Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2116974119
• All: 1 reference(s)