Vav1^{tm1.1(Myo1f)Tpal}
Targeted Allele Detail

Summary

• Symbol: Vav1^{tm1.1(Myo1f)Tpal}
• Name: vav 1 oncogene; targeted mutation 1.1, Teresa Palomero
• MGI ID: MGI:7431424
• Synonyms: Vav1^{co-Vav1-Myo1f}
• Gene: Vav1 Location: Chr17:57586100-57635031 bp, + strand Genetic Position: Chr17, 29.76 cM
• Alliance: Vav1^{tm1.1(Myo1f)Tpal} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325006
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Vav1^{tm1.1(Myo1f)Tpal} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Myo1f (MGI:107711)	Results in expression of a Vav1/Myo1 oncogenic fusion.

• Mutation details: A minigene containing floxed Vav1 exon 25-27 and FRT-flanked neomycin selection cassette were introduced in intron 24 and the downstream exons 25 and 26 were replaced with Vav1 exons 25-27 fused to the cDNA sequence corresponding to exons 25-28 of Myo1f. Exon 24 of Vav1 is spliced into the exon 25-27 minigene resulting in expression of a wild-type Vav1 mRNA. The neomycin cassette was removed via flp-mediated recombination. Cre recombinase expression is needed to remove the floxed exon 25-27 minigene to lead to exon 24 being spliced into the Vav1 exon 25-27Myo1f exons 25-28 minigene, which will result in the expression of the Vav1-Myo1f oncogenic fusion. (J:325006)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vav1 Mutation: 59 strains or lines available

References

• Original: J:325006 Cortes JR, et al., Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma. Cell Rep. 2022 Apr 19;39(3):110695
• All: 1 reference(s)