Gt(ROSA)26Sor^{em1(Btg1)Meln}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(Btg1)Meln}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Ari Melnick
• MGI ID: MGI:7430731
• Synonyms: R26^lsl.Btg1wt
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(Btg1)Meln} page

Mutation origin

• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting vector inserted a loxp flanked stop cassette in intron1 of the Rosa26 locus followed immediately by a wild-type murine Btg1 cDNA-T2A-Luciferase sequence. The allele was generated by introducing a single-nucleotide substitution to revert the Q36H mutation of the cDNA sequence in Gt(ROSA)26Sor^{tm1(Btg1*)Meln} to a wild-type sequence by CRISPR-Cas9-mediated genome targeting. (J:332796)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:332796 Mlynarczyk C, et al., BTG1 mutation yields supercompetitive B cells primed for malignant transformation. Science. 2023 Jan 20;379(6629):eabj7412
• All: 1 reference(s)