Neurod2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Neurod2^{tm1c(KOMP)Wtsi}
• Name: neurogenic differentiation 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7428644
• Synonyms: Neurod2^flox
• Gene: Neurod2 Location: Chr11:98216241-98220471 bp, - strand Genetic Position: Chr11, 61.75 cM
• Alliance: Neurod2^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0422_5_B06
• Germline Transmission: Earliest citation of germline transmission: J:313940
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 98216249 of Chromosome 11 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 98219618. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene and generated a "conditional ready" allele was created in which exon 2 is floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:313940)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Neurod2 Mutation: 23 strains or lines available

References

• Original: J:313940 Runge K, et al., Disruption of NEUROD2 causes a neurodevelopmental syndrome with autistic features via cell-autonomous defects in forebrain glutamatergic neurons. Mol Psychiatry. 2021 Jun 29
• All: 1 reference(s)