Lrpprc^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Lrpprc^{tm1c(KOMP)Wtsi}
• Name: leucine-rich PPR-motif containing; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7428577
• Synonyms: Lrpprc^loxp
• Gene: Lrpprc Location: Chr17:85012675-85098214 bp, - strand Genetic Position: Chr17, 55.02 cM
• Alliance: Lrpprc^{tm1c(KOMP)Wtsi} page
• IMPC: Lrpprc gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243637
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85080229 of Chromosome 17 upstream of the critical exons 4-5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 4-5 at position 85081240. The critical exons 4-5 are thus flanked by loxP sites. The neomycin resistance cassette and lacZ were removed via flp-mediated recombination generating a conditional ready allele with exons 4-5 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:243637)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lrpprc Mutation: 60 strains or lines available

References

• Original: J:243637 Cuillerier A, et al., Loss of hepatic LRPPRC alters mitochondrial bioenergetics, regulation of permeability transition and trans-membrane ROS diffusion. Hum Mol Genet. 2017 Aug 15;26(16):3186-3201
• All: 3 reference(s)