Gt(ROSA)26Sor^{em2(CAG-Map2k5*S311D*T315D)Pkma}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-Map2k5*S311D*T315D)Pkma}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Pawel K Mazur
• MGI ID: MGI:7427559
• Synonyms: Rosa26-LSL-Mek5^S311D/T315D
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-Map2k5*S311D*T315D)Pkma} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A CAG-loxP-Stop-loxP-V5-Map2k5 cDNA containing a serine to aspartate substitution at amino acid 311 (p.S311D) and a threonine to aspartate substitution at amino acid 315 (p.T315D)-polyA cassette was inserted into intron 1 via CRISPR/Cas9 technology. (J:328655)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:328655 Lukinovic V, et al., SMYD3 Impedes Small Cell Lung Cancer Sensitivity to Alkylation Damage through RNF113A Methylation-Phosphorylation Cross-talk. Cancer Discov. 2022 Sep 2;12(9):2158-2179
• All: 1 reference(s)