Arl2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arl2^{tm1c(KOMP)Wtsi}
• Name: ADP-ribosylation factor-like 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7427478
• Gene: Arl2 Location: Chr19:6184419-6191167 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Arl2^{tm1c(KOMP)Wtsi} page
• IMPC: Arl2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332516
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 6187250 of Chromosome 19 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 6188304. The critical exons are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:332516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arl2 Mutation: 16 strains or lines available

References

• Original: J:332516 Gerstner CD, et al., Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development. Cells. 2022 Dec 30;12(1)
• All: 2 reference(s)