Mthfd2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mthfd2^{tm1c(EUCOMM)Wtsi}
• Name: methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7427381
• Gene: Mthfd2 Location: Chr6:83282673-83302890 bp, - strand Genetic Position: Chr6, 35.94 cM
• Alliance: Mthfd2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mthfd2 gene page

Mutation origin

• Mutant Cell Lines: EPD0752_2_A07, EPD0752_2_F07
• Germline Transmission: Earliest citation of germline transmission: J:324877
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 83290107 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 83290957. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534, J:324877)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mthfd2 Mutation: 23 strains or lines available

References

• Original: J:324877 Sugiura A, et al., MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function. Immunity. 2022 Jan 11;55(1):65-81.e9
• All: 3 reference(s)