Pdzd8^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pdzd8^{tm1c(EUCOMM)Wtsi}
• Name: PDZ domain containing 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7427380
• Gene: Pdzd8 Location: Chr19:59285610-59334212 bp, - strand Genetic Position: Chr19, 54.65 cM
• Alliance: Pdzd8^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pdzd8 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324881
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 59303414 of Chromosome 19 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 59304251. The critical exon is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:324881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdzd8 Mutation: 62 strains or lines available

References

• Original: J:324881 O'Hare JK, et al., Compartment-specific tuning of dendritic feature selectivity by intracellular Ca(2+) release. Science. 2022 Mar 18;375(6586):eabm1670
• All: 2 reference(s)