Nnat^tm1Posp
Targeted Allele Detail

Summary

• Symbol: Nnat^tm1Posp
• Name: neuronatin; targeted mutation 1, J Andrew Pospislik
• MGI ID: MGI:7425291
• Gene: Nnat Location: Chr2:157401998-157404442 bp, + strand Genetic Position: Chr2, 78.4 cM, cytoband H1
• Alliance: Nnat^tm1Posp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230648
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 157402687 of Chromosome 2 upstream of exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 3 at position 157404689. The critical exons are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:230648)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nnat Mutation: 15 strains or lines available

References

• Original: J:230648 Dalgaard K, et al., Trim28 Haploinsufficiency Triggers Bi-stable Epigenetic Obesity. Cell. 2016 Jan 28;164(3):353-64
• All: 1 reference(s)