Gt(ROSA)26Sor^em1.1Amde
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^em1.1Amde
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Anne M Delany
• MGI ID: MGI:7425282
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^em1.1Amde page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A construct containing a CAG promoter, floxed stop cassette, tdTomato CDS, 3X Mir29-3p tough decoy, and WPRE was inserted in the gene via CRISPR-cas9 mediated recombination. The Mir29-3p tough decoy contains binding sites for the members of the Mir29 family including Mir29a, Mir29b-1, Mir29b-2, and Mir29c. Cre mediated recombination removed the floxed stop cassette.qRT-PCR analysis found a 30-60% decrease in levels in the calvaria of mice carrying the allele. (J:313732)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:313732 Hrdlicka HC, et al., Inhibition of miR-29-3p isoforms via tough decoy suppresses osteoblast function in homeostasis but promotes intermittent parathyroid hormone-induced bone anabolism. Bone. 2021 Feb;143:115779
• All: 1 reference(s)