Adar^tm1.1Cya
Targeted Allele Detail

Summary

• Symbol: Adar^tm1.1Cya
• Name: adenosine deaminase, RNA-specific; targeted mutation 1.1, Cyagen Biosciences
• MGI ID: MGI:7425019
• Synonyms: Adar1^fl-mZalpha
• Gene: Adar Location: Chr3:89622329-89660753 bp, + strand Genetic Position: Chr3, 39.19 cM, cytoband F2
• Alliance: Adar^tm1.1Cya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323768
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A construct containing a loxP site, exon 2, a Rox-flanked neomycin resistance gene, lox2272 site, exon 2 in an inverted orientation containing four nucleotide substitutions c.523-5 AAT>GCT and c.535-7 TAC>GCC resulting in an asparagine to alanine substitution at amino acid 175 and a tyrosine to alanine substitution at amino acid 179 (p.Asn175Ala, p.Tyr179Ala), loxP site, and lox2272 replaced exon 2. These residues are essential for Z-DNA (Asn175) and Z-RNA (Tyr179) binding. The neomycin resistance gene was removed. (J:323768)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adar Mutation: 83 strains or lines available

References

• Original: J:323768 Tang Q, et al., Adenosine-to-inosine editing of endogenous Z-form RNA by the deaminase ADAR1 prevents spontaneous MAVS-dependent type I interferon responses. Immunity. 2021 Sep 14;54(9):1961-1975.e5
• All: 1 reference(s)