Pold3^tm1.2Ofc
Targeted Allele Detail

Summary

• Symbol: Pold3^tm1.2Ofc
• Name: polymerase (DNA-directed), delta 3, accessory subunit; targeted mutation 1.2, Oscar Fernandez-Capetillo
• MGI ID: MGI:7423622
• Gene: Pold3 Location: Chr7:99731317-99770709 bp, - strand Genetic Position: Chr7, 54.32 cM, cytoband F1
• Alliance: Pold3^tm1.2Ofc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236984
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A EUCOMM targeting vector was used to create this conditional-ready floxed allele. The vector places an FRT site, splice acceptor site, IRES, lacZ cassette, loxP site, neomycin resistance gene cassette, second FRT site and second loxP site upstream of exon 2 and a third loxP site downstream of exon 2. Flp-mediated recombination removed the lacZ and neomycin cassette. Exon 2 was deleted via cre-mediated recombination in the germline. (J:236984)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pold3 Mutation: 36 strains or lines available

References

• Original: J:236984 Murga M, et al., POLD3 Is Haploinsufficient for DNA Replication in Mice. Mol Cell. 2016 Sep 1;63(5):877-83
• All: 1 reference(s)